soluble trail peprotech  (PeproTech)

Journal: British Journal of Cancer

Article Title: In vitro sensitivity testing of minimally passaged and uncultured gliomas with TRAIL and/or chemotherapy drugs

doi: 10.1038/sj.bjc.6604459

Figure Lengend Snippet: Chemotherapy drugs and doses used in this study

Article Snippet: We used soluble TRAIL (Peprotech, Rocky Hill, CT, USA) at concentrations commonly employed in vitro (1 μ g ml −1 and 100 ng ml −1 ).

Techniques: Clinical Proteomics, Concentration Assay

Journal: British Journal of Cancer

Article Title: In vitro sensitivity testing of minimally passaged and uncultured gliomas with TRAIL and/or chemotherapy drugs

doi: 10.1038/sj.bjc.6604459

Figure Lengend Snippet: Propidium iodide uptake assay of glioma cell sensitivity to combination treatments. Cells from the indicated early passage glioma cell lines or ex vivo gliomas were incubated in vitro with soluble TRAIL at 100 ng ml −1 (+) or 1000 ng ml −1 (++) alone or together with temozolomide (13.7 μ g ml −1 ) or cisplatin (54 μ g ml −1 ) for 48 h. Flow cytometry measurement of propidium iodide exclusion was used to quantitate the proportion of surviving cells. The areas of the circles denote survival following each treatment. Small circles indicate efficient killing, large circles reflect survival.

Article Snippet: We used soluble TRAIL (Peprotech, Rocky Hill, CT, USA) at concentrations commonly employed in vitro (1 μ g ml −1 and 100 ng ml −1 ).

Techniques: Ex Vivo, Incubation, In Vitro, Flow Cytometry